Morphological, molecular and FTIR spectroscopic analysis during the differentiation of kidney cells from pluripotent stem cells
Biol. Res
;
50: 14, 2017. tab, graf
Artigo
em Inglês
| LILACS
| ID: biblio-838965
ABSTRACT
BACKGROUND:
Kidney diseases are a global health problem. Currently, over 2 million people require dialysis or transplant which are associated with high morbidity and mortality; therefore, new researches focused on regenerative medicine have been developed, including the use of stem cells.RESULTS:
In this research, we generate differentiated kidney cells (DKCs) from mouse pluripotent stem cells (mPSCs) analyzing their morphological, genetic, phenotypic, and spectroscopic characteristics along differentiation, highlighting that there are no reports of the use of Fourier transform infrared (FTIR) spectroscopy to characterize the directed differentiation of mPSCs to DKCs. The genetic and protein experiments proved the obtention of DKCs that passed through the chronological stages of embryonic kidney development. Regarding vibrational spectroscopy analysis by FTIR, bands related with biomolecules were shown on mPSCs and DKCs spectra, observing distinct differences between cell lineages and maturation stages. The second derivative of DKCs spectra showed changes in the protein bands compared to mPSCs. Finally, the principal components analysis obtained from FTIR spectra allowed to characterize chemical and structurally mPSCs and their differentiation process to DKCs in a rapid and non-invasive way.CONCLUSION:
Our results indicated that we obtained DKCs from mPSCs, which passed through the chronological stages of embryonic kidney development. Moreover, FTIR spectroscopy resulted in a non-invasive, rapid and precise technic that together with principal component analysis allows to characterize chemical and structurally both kind of cells and also discriminate and determine different stages along the cell differentiation process.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Diferenciação Celular
/
Espectroscopia de Infravermelho com Transformada de Fourier
/
Células-Tronco Pluripotentes
/
Rim
Limite:
Animais
Idioma:
Inglês
Revista:
Biol. Res
Assunto da revista:
Biologia
Ano de publicação:
2017
Tipo de documento:
Artigo
País de afiliação:
México
Instituição/País de afiliação:
Instituto Politécnico Nacional/MX
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