Protein carbonyl content: a novel biomarker for aging in HIV/AIDS patients
Braz. j. infect. dis
;
21(1): 35-41, Jan.-Feb. 2017. tab, graf
Artigo
em Inglês
| LILACS
| ID: biblio-839181
ABSTRACT
Abstract Background:
The major complications of “treated” Human Immunodeficiency Virus (HIV) infection are cardiovascular disease, malignancy, renal disease, liver disease, bone disease, and perhaps neurological complications, which are phenomena of the normal aging process occurring at an earlier age in the HIV-infected population. The present study is aimed to explore protein carbonyl content as a biomarker for detecting oxidative DNA damage induced ART toxicity and/or accelerated aging in HIV/AIDS patients.Objective:
To investigate the potential of carbonyl content as a biomarker for detecting oxidative Deoxyribonucleic acid (DNA) damage induced Antiretroviral Theraphy (ART) toxicity and/or accelerated aging in HIV/AIDS patients.Methods:
In this case–control study a total 600 subjects were included. All subjects were randomly selected and grouped as HIV-negative (control group) (n = 300), HIV-infected ART naive (n = 100), HIV-infected on first line ART (n = 100), and HIV-infected on second line ART (n = 100). Seronegative control subjects were age- and sex-matched with the ART naive patients and the two other groups. Carbonyl protein was determined by the method described in Levine et al. DNA damage marker 8-OH-dG was determined using 8-hydroxy-2-deoxy Guanosine StressXpress ELA Kit by StressMarq Biosciences.Results:
Protein carbonyl content levels and oxidative DNA damage were significantly higher (p < 0.05) in HIV-infected patients on second line ART and HIV-infected patients on first line ART than ART naive patients and controls. In a linear regression analysis, increased protein carbonyl content was positively associated with increased DNA damage (OR 0.356; 95% CI 0.287–0.426) p < 0.05.Conclusions:
Carbonyl content may has a role as a biomarker for detecting oxidative DNA damage induced ART toxicity and/or accelerated aging in HIV/AIDS patients. Larger studies are warranted to elucidate the role of carbonyl content as a biomarker for premature aging in HIV/AIDS patients.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Dano ao DNA
/
Envelhecimento
/
Síndrome de Imunodeficiência Adquirida Felina
/
Síndrome da Imunodeficiência Adquirida
/
Terapia Antirretroviral de Alta Atividade
/
Desoxiguanosina
/
Carbonilação Proteica
Tipo de estudo:
Estudo observacional
/
Fatores de risco
Limite:
Animais
/
Feminino
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Braz. j. infect. dis
Assunto da revista:
Doenças Transmissíveis
Ano de publicação:
2017
Tipo de documento:
Artigo
País de afiliação:
Índia
Instituição/País de afiliação:
Grant Government Medical College and Sir JJ Group of Hospitals/IN
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