Grandisin induces apoptosis in leukemic K562 cells
Braz. J. Pharm. Sci. (Online)
;
53(1): e15210, 2017. graf
Artigo
em Inglês
| LILACS
| ID: biblio-839446
ABSTRACT
Abstract In this study, the potential antileukemic activity of grandisin, a lignan extracted from Piper solmsianum, was evaluated against the leukemic line K562. The cytotoxicity of grandisin (0.018 to 2.365 µM) was evaluated in K562 and normal peripheral blood lymphocytes by Trypan Blue Exclusion and MTT methods after 48h exposure to the drug. In both methods, cellular viability was concentration-dependent and the IC50 values were lower than 0.85µM. Analysis of K562 cells after treatment with grandisin showed that the cell cycle was arrested in the G1 phase with a 12.31% increase, while both S and G2 phases decreased. Morphological studies conducted after the exposure of K562 to grandisin revealed changes consistent with the apoptosis process, which was confirmed by anexin V stain and caspase activation. Thus, lignan grandisin showed antileukemic activities against the K562 cell line and the cell death process occurred via apoptosis.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Regulação Leucêmica da Expressão Gênica
/
Lignanas
/
Células K562
/
Fator de Indução de Apoptose
Idioma:
Inglês
Revista:
Braz. J. Pharm. Sci. (Online)
Assunto da revista:
Farmacologia
/
Teraputica
/
Toxicologia
Ano de publicação:
2017
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Federal University of Goiás/BR
/
University of São Paulo/BR
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