Simultaneous determination of atorvastatin and ezetimibe from combined pharmaceutical products by micellar electrokinetic capillary chromatography
Braz. J. Pharm. Sci. (Online)
;
53(1): e16122, 2017. tab, graf
Artigo
em Inglês
| LILACS
| ID: biblio-839447
ABSTRACT
Abstract A rapid and sensitive micellar electrokinetic capillary chromatography method with UV photodiode-array detection was developed for the simultaneous determination of atorvastatin and ezetimibe in fixed dose drug combination. Experimental conditions such as buffer concentration and pH, surfactant concentration, system temperature, applied voltage, injection parameters were optimized in order to improve the efficiency of the separation. The best results were obtained when using fused silica capillary (48 cm length X 50 µm ID) and 25 mM borate buffer electrolyte at pH 9.3 containing 25 mM SDS, + 30 kV applied voltage, 20 ºC system temperature. The separation was achieved in approximately 2 minutes, with a resolution of 7.02, the order of migration being atorvastatin followed by ezetimibe. The analytical performance of the method was verified with regard to linearity, precision, robustness and the limit of detection and quantification were calculated.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Cromatografia Capilar Eletrocinética Micelar
/
Ezetimiba
/
Atorvastatina
Idioma:
Inglês
Revista:
Braz. J. Pharm. Sci. (Online)
Assunto da revista:
Farmacologia
/
Teraputica
/
Toxicologia
Ano de publicação:
2017
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Romênia
Instituição/País de afiliação:
Gedeon Richter Romania/RO
/
University of Medicine and Pharmacy/RO
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