Stability indicating RP-HPLC method for simultaneous determination of gatifloxacin and dexamethasone in binary combination
Braz. J. Pharm. Sci. (Online)
;
53(1): e15177, 2017. tab, graf
Artigo
em Inglês
| LILACS
| ID: biblio-839449
ABSTRACT
Abstract In this study, conditions were optimized for development of a simple RP-HPLC method for simultaneous analysis of gatifloxacin and dexamethasone in different matrices like pharmaceuticals, human serum and urine. Good separation of gatifloxacin and dexamethasone from the induced degradation products was accomplished using C8 as stationary phase; 0.02 M phosphate buffer (pH 3.0) and methanol (4258 v/v) as mobile phase. The concentration was measured with DAD at 270 nm. Linearity was observed in the range of 0.000040-0.000280 mol/L for gatifloxacin (r2≥0.999) and 0.000013-0.000091 mol/L for dexamethasone (r2≥0.999). Both the analyte peaks were completely separated from the peaks of induced degradation products as indicated by the peak purity index (≥0.9999 for both analytes). The optimized method is recommended to be used for concurrent analysis of gatifloxacin and dexamethasone in different matrices.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Cromatografia Líquida de Alta Pressão
/
Estabilidade de Medicamentos
/
Cromatografia de Fase Reversa
/
Antibacterianos
Idioma:
Inglês
Revista:
Braz. J. Pharm. Sci. (Online)
Assunto da revista:
Farmacologia
/
Teraputica
/
Toxicologia
Ano de publicação:
2017
Tipo de documento:
Artigo
País de afiliação:
Paquistão
Instituição/País de afiliação:
Government College University/PK
/
Medipharm Pharmaceuticals Kot Lakhpat/PK
/
Queen Marry College/PK
/
University of Gujra/PK
/
University of Gujrat/PK
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