Application of HPLC-DAD for the quantification of Lycorine in Galanthus elwesii Hook
Braz. J. Pharm. Sci. (Online)
;
53(1): e15063, 2017. tab, graf
Artigo
em Inglês
| LILACS
| ID: biblio-839465
ABSTRACT
ABSTRACT In the present study, a reversed-phase high-performance liquid chromatographic method has been used for the quantitative determination of lycorine in the aerial parts and bulbs of G. elwesii Hook. A simple method for the extraction of lycorine in low mass plant samples was employed utilizing pre-packed columns with diatomaceous earth (Extrelut(r)). The chromatographic separation was performed using an isocratic system with a mobile phase of trifluoroacetic acid-water-acetonitrile (0.0192.57.5, v/v/v) applied at a flow rate 1 mL min-1 using diode array detector. The content of lycorine in the bulbs and aerial parts of G. elwesii collected from Demirci (Manisa) was found as 0.130 and 0.162 %, respectively. Additionally, in the bulbs of the specimens collected from Sogucak (Balikesir), lycorine was quantified as 0.055 %, whereas in the aerial parts, it was determined as 0.006 %. The method was validated partially with respect to system specificity, linearity, accuracy, precision, limits of detection (LOD) and quantitation (LOQ). Validation procedures displayed that the method was specific, accurate and precise.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Cromatografia Líquida de Alta Pressão
/
Alcaloides
/
Amaryllidaceae
Tipo de estudo:
Estudos de avaliação
Idioma:
Inglês
Revista:
Braz. J. Pharm. Sci. (Online)
Assunto da revista:
Farmacologia
/
Teraputica
/
Toxicologia
Ano de publicação:
2017
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Turquia
Instituição/País de afiliação:
Ege University/TR
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