An innovative, simple, fast, and less toxic high-performance liquid chromatographic method for determination of prednisone in capsules
Braz. J. Pharm. Sci. (Online)
;
53(2): e16064, 2017. tab, graf
Artigo
em Inglês
| LILACS
| ID: biblio-839484
ABSTRACT
ABSTRACT Prednisone is an anti-inflammatory steroid drug widely used in clinical practice. However, no high-performance liquid chromatographic (HPLC) method has been described in the literature for the determination of prednisone in capsules until now. Thus, an HPLC method was developed using a C18 (250x4.0, 5 µm) column, with methanolwater (7030) as mobile phase at a flow rate of 1 mL/min and detection at 240 nm. The developed method was validated following current Brazilian legislation. Additionally, linearity was assessed by evaluating the assumptions of normality, homoscedasticity, and independency of residuals, and the fit to the linear model. The method showed linearity (r2>0.99) over the range of 14.0-26.0 µg/mL, selectivity, precision (RSD<2.0%), robustness, and accuracy (average recovery of 100.05%). The chromatographic procedure was applied for assay and uniformity content determination of three different batches of prednisone capsules, showing to be suitable for their quality control.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Cápsulas
/
Prednisona
/
Cromatografia Líquida de Alta Pressão
Idioma:
Inglês
Revista:
Braz. J. Pharm. Sci. (Online)
Assunto da revista:
Farmacologia
/
Teraputica
/
Toxicologia
Ano de publicação:
2017
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Federal University of Minas Gerais/BR
/
Oswaldo Cruz Foundation/BR
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