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Oral mucosa: an alternative epidermic cell source to develop autologous dermal-epidermal substitutes from diabetic subjects
GUZMÁN-URIBE, Daniela; ALVARADO-ESTRADA, Keila Neri; PIERDANT-PÉREZ, Mauricio; TORRES-ÁLVAREZ, Bertha; SÁNCHEZ-AGUILAR, Jesus Martin; ROSALES-IBÁÑEZ, Raúl.
  • GUZMÁN-URIBE, Daniela; Universidad Autónoma de San Luis Potosí. Facultad de Estomatología. San Luis Potosí. MX
  • ALVARADO-ESTRADA, Keila Neri; Universidad Autónoma de San Luis Potosí. Facultad de Estomatología. San Luis Potosí. MX
  • PIERDANT-PÉREZ, Mauricio; Universidad Autónoma de San Luis Potosí. Facultad de Estomatología. San Luis Potosí. MX
  • TORRES-ÁLVAREZ, Bertha; Universidad Autónoma de San Luis Potosí. Facultad de Estomatología. San Luis Potosí. MX
  • SÁNCHEZ-AGUILAR, Jesus Martin; Universidad Autónoma de San Luis Potosí. Facultad de Estomatología. San Luis Potosí. MX
  • ROSALES-IBÁÑEZ, Raúl; Universidad Autónoma de San Luis Potosí. Facultad de Estomatología. San Luis Potosí. MX
J. appl. oral sci ; 25(2): 186-195, Mar.-Apr. 2017. tab, graf
Artigo em Inglês | LILACS, BBO | ID: biblio-841186
ABSTRACT
Abstract Oral mucosa has been highlighted as a suitable source of epidermal cells due to its intrinsic characteristics such as its higher proliferation rate and its obtainability. Diabetic ulcers have a worldwide prevalence that is variable (1%-11%), meanwhile treatment of this has been proven ineffective. Tissue-engineered skin plays an important role in wound care focusing on strategies such autologous dermal-epidermal substitutes. Objective The aim of this study was to obtain autologous dermal-epidermal skin substitutes from oral mucosa from diabetic subjects as a first step towards a possible clinical application for cases of diabetic foot. Material and Methods Oral mucosa was obtained from diabetic and healthy subjects (n=20 per group). Epidermal cells were isolated and cultured using autologous fibrin to develop dermal-epidermal in vitro substitutes by the air-liquid technique with autologous human serum as a supplement media. Substitutes were immunocharacterized with collagen IV and cytokeratin 5-14 as specific markers. A Student´s t- test was performed to assess the differences between both groups. Results It was possible to isolate epidermal cells from the oral mucosa of diabetic and healthy subjects and develop autologous dermal-epidermal skin substitutes using autologous serum as a supplement. Differences in the expression of specific markers were observed and the cytokeratin 5-14 expression was lower in the diabetic substitutes, and the collagen IV expression was higher in the diabetic substitutes when compared with the healthy group, showing a significant difference. Conclusion Cells from oral mucosa could be an alternative and less invasive source for skin substitutes and wound healing. A difference in collagen production of diabetic cells suggests diabetic substitutes could improve diabetic wound healing. More research is needed to determine the crosstalk between components of these skin substitutes and damaged tissues.
Assuntos


Texto completo: DisponíveL Índice: LILACS (Américas) Assunto principal: Pele Artificial / Transplante de Células / Diabetes Mellitus Tipo 2 / Epiderme / Células Epiteliais / Mucosa Bucal Tipo de estudo: Estudo observacional / Fatores de risco Limite: Adulto / Idoso / Feminino / Humanos / Masculino Idioma: Inglês Revista: J. appl. oral sci Assunto da revista: Odontologia Ano de publicação: 2017 Tipo de documento: Artigo País de afiliação: México Instituição/País de afiliação: Universidad Autónoma de San Luis Potosí/MX

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Texto completo: DisponíveL Índice: LILACS (Américas) Assunto principal: Pele Artificial / Transplante de Células / Diabetes Mellitus Tipo 2 / Epiderme / Células Epiteliais / Mucosa Bucal Tipo de estudo: Estudo observacional / Fatores de risco Limite: Adulto / Idoso / Feminino / Humanos / Masculino Idioma: Inglês Revista: J. appl. oral sci Assunto da revista: Odontologia Ano de publicação: 2017 Tipo de documento: Artigo País de afiliação: México Instituição/País de afiliação: Universidad Autónoma de San Luis Potosí/MX