Effect of laser therapy on laryngeal carcinoma cell proliferation(H Ep-2)

ABSTRACT

Objective: to evaluate the effect of 660nm and 780 nm laser therapy, with dose of 6.3 J/cm² and 25 mW power, either associated with nutritional stress or not, on laryngeal epidermoid carcinoma cell proliferation (H.Ep. 2). Material and Methods: the H.Ep.2 cells were placed in a culture flask and frozen in fetal bovine serum (FBS) at -80°C, with different concentrations of the medium 5% and 10%. Laser therapy was started 24 h after cell subculturing and performed at time intervals of 6, 12, 24, 48 and 72 hours after the first irradiation, in a period of five days. After this, cell viability was verified using the MTT method. Means and standard deviation were obtained, and the Student's-t and F (ANOVA) statistical tests were used, with Tukey or Tamanhe comparisons. Results: The group subjected to 780 nm laser showed significant differences (p 0.05). The time influenced the growth of all cultures. Conclusion: low level laser interfered in cell proliferation and this effect could be determined by the wavelength used and the nutritional status of the cells.