The influence of allopurinol and post-conditioning on lung injuries induced by lower-limb ischemia and reperfusion in Wistar rats
Acta cir. bras
; 32(9): 746-754, Sept. 2017. tab, graf
Article
em En
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| ID: biblio-886234
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BR1.1
ABSTRACT
Abstract Purpose:
To analyze the effects of allopurinol and of post-conditioning on lung injuries induced by lower-limb ischemia and reperfusion.Methods:
Thirty rats were used. They were divided in 5 groups (1) group A abdominal aortic dissection only, (2) group B ischemia and reperfusion, (3) group C administered allopurinol (100mg/Kg) a few hours before procedure, (4) group D post-conditioned and (5) group E administered allopurinol and post-conditioned. With the exception of group A, all groups were submitted to infrarenal aortic ischemia for 2 hours, and reperfusion for 72 hours. After euthanasia, lungs were removed for histological analysis. They were graded under two scores pulmonary injury (neutrophil infiltration, interstitial edema, vascular congestion, and destruction of lung architecture) and lymphocytic score (neutrophil infiltration, lymphoid aggregate and secondary follicle).Results:
On the pulmonary injury score, the degree of injury was smaller than in groups D and E, when compared to group B, p<0.05. Group C did not obtain the same result (p>0,05). On the lymphocytic score, there was no statistic difference among groups, p>0.05.Conclusion:
Both post-conditioning and the combination of allopurinol and post-conditioning were effective in remote lung protection induced by lower-limbs I/R. When used in isolation, allopurinol showed no protective effect.Palavras-chave
Texto completo:
1
Índice:
LILACS
Assunto principal:
Traumatismo por Reperfusão
/
Alopurinol
/
Lesão Pulmonar
/
Pós-Condicionamento Isquêmico
Tipo de estudo:
Etiology_studies
Limite:
Animals
Idioma:
En
Revista:
Acta cir. bras
Assunto da revista:
CIRURGIA GERAL
/
Procedimentos Cir£rgicos Operat¢rios
Ano de publicação:
2017
Tipo de documento:
Article