Development of a real-time PCR assay for the detection of the golden mussel ( Limnoperna fortunei, Mytilidae) in environmental samples
An. acad. bras. ciênc
;
89(2): 1041-1045, Apr.-June 2017. tab, graf
Artigo
em Inglês
| LILACS
| ID: biblio-886687
ABSTRACT
ABSTRACT The golden mussel, Limnoperna fortunei, is among the most devastating invasive species in freshwater habitats worldwide, leading to severe environmental disturbances and economic losses. Therefore, management efforts would be greatly improved by methods that efficiently detect and quantify the abundance of the golden mussel in freshwater habitats, particularly in early stages of colonization. In this study, we describe a highly-sensitive real-time PCR assay targeting a 100-bp region of the COI mitochondrial gene of the golden mussel. The method was able to detect as little as 0.225 pg of target DNA. This assay represents an important contribution to surveillance methods, as well as to optimize field measures to contain and manage populations of the golden mussel in its introduced range.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Mytilidae
/
Espécies Introduzidas
/
Reação em Cadeia da Polimerase em Tempo Real
Tipo de estudo:
Estudo diagnóstico
/
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
An. acad. bras. ciênc
Assunto da revista:
Ciência
Ano de publicação:
2017
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Universidade Federal do Paraná/BR
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