Cryopreservation of banana's cv Grand Naine in vitro rhizomes
An. acad. bras. ciênc
;
90(1): 495-507, Mar. 2018. tab, graf
Artigo
em Inglês
| LILACS
| ID: biblio-886920
ABSTRACT
ABSTRACT The preservation of banana genetic material is usually performed through seedlings. However, most banana cultivars do not produce seed and are propagated vegetatively. Therefore, cryopreservation is a feasible technique that allows the preservation of banana genotypes indefinitely. For the success of cryopreservation protocols, the selection of cryoprotectants and pre-freezing techniques are important factor. Therefore, the objective of this study was to verify the effects of different cryoprotectants with and without 1% phloroglucinol and pre-cooling periods on the development of a protocol for cryopreservation of in vitro rhizomes ofMusa accuminata(AAA) cv Grand Naine banana. The addition of 1% phloroglucinol to the cryoprotective solutions, such as PVS2 enhanced recovery of cryopreserved banana rhizomes. In addition, pre-cooling of explants in ice for 3 hours in PVS2 + 1% of phloroglucinol allowed efficient cryopreservation of banana rhizomes, followed by successful recovery and regeneration of in vitro shoots of banana cv Grand Naine.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Floroglucinol
/
Criopreservação
/
Crioprotetores
/
Musa
/
Rizoma
Tipo de estudo:
Estudos de avaliação
Idioma:
Inglês
Revista:
An. acad. bras. ciênc
Assunto da revista:
Ciência
Ano de publicação:
2018
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Empresa de Pesquisa Agropecuária de Minas Gerais/BR
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