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IL-10 down-regulates the expression of survival associated gene hspX of Mycobacterium tuberculosis in murine macrophage
Jee, Babban; Sharma, Pawan; Katoch, Kiran; Joshi, Beenu; Awasthi, Sudhir Kumar.
  • Jee, Babban; National JALMA Institute for Leprosy and other Mycobacterial Diseases (ICMR). Department of Microbiology and Molecular Biology. Agra. IN
  • Sharma, Pawan; National JALMA Institute for Leprosy and other Mycobacterial Diseases (ICMR). Department of Microbiology and Molecular Biology. Agra. IN
  • Katoch, Kiran; National JALMA Institute for Leprosy and other Mycobacterial Diseases (ICMR). Department of Microbiology and Molecular Biology. Agra. IN
  • Joshi, Beenu; National JALMA Institute for Leprosy and other Mycobacterial Diseases (ICMR). Department of Microbiology and Molecular Biology. Agra. IN
  • Awasthi, Sudhir Kumar; National JALMA Institute for Leprosy and other Mycobacterial Diseases (ICMR). Department of Microbiology and Molecular Biology. Agra. IN
Braz. j. infect. dis ; 21(4): 386-390, July-Aug. 2017. graf
Artigo em Inglês | LILACS | ID: biblio-888886
ABSTRACT
Abstract Mycobacterium tuberculosis (MTB) adopts a special survival strategy to overcome the killing mechanism(s) of host immune system. Amongst the many known factors, small heat shock protein 16.3 (sHSP16.3) of MTB encoded by gene hspX has been reported to be critical for the survival of MTB. In the present study, the effect of recombinant murine interferon-gamma (rmIFN-γ) and recombinant murine interleukin-10 (rmIL-10) on the expression of gene hspX of MTB in murine macrophage RAW264.7 has been investigated. By real-time RT-PCR, it was observed that three increasing concentrations (5, 25 and 50 ng/ml) of rmIFN-γ significantly up-regulated the expression of hspX whereas similar concentrations of rmIL-10 (5, 25 and 50 ng/ml) significantly down-regulated the hspX expression. This effect was not only dependent on the concentration of the stimulus but this was time-dependent as well. A contrasting pattern of hspX expression was observed against combinations of two different concentrations of rmIFN-γ and rmIL-10. The study results suggest that rIL-10 mediated down-regulation of hspX expression, in the presence of low concentration of rIFN-γ, could be used as an important strategy to decrease the dormancy of MTB in its host and thus making MTB susceptible to the standard anti-mycobacterial therapy used for treating tuberculosis. However, as these are only preliminary results in the murine cell line model, this hypothesis needs to be first validated in human cell lines and subsequently in animal models mimicking the latent infection using clinical isolates of MTB before considering the development of modified regimens for humans.
Assuntos


Texto completo: DisponíveL Índice: LILACS (Américas) Assunto principal: Proteínas de Bactérias / Regulação Bacteriana da Expressão Gênica / Interferon gama / Interleucina-10 / Macrófagos / Mycobacterium tuberculosis / Antígenos de Bactérias Tipo de estudo: Estudo prognóstico / Fatores de risco Idioma: Inglês Revista: Braz. j. infect. dis Assunto da revista: Doenças Transmissíveis Ano de publicação: 2017 Tipo de documento: Artigo País de afiliação: Índia Instituição/País de afiliação: National JALMA Institute for Leprosy and other Mycobacterial Diseases (ICMR)/IN

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Texto completo: DisponíveL Índice: LILACS (Américas) Assunto principal: Proteínas de Bactérias / Regulação Bacteriana da Expressão Gênica / Interferon gama / Interleucina-10 / Macrófagos / Mycobacterium tuberculosis / Antígenos de Bactérias Tipo de estudo: Estudo prognóstico / Fatores de risco Idioma: Inglês Revista: Braz. j. infect. dis Assunto da revista: Doenças Transmissíveis Ano de publicação: 2017 Tipo de documento: Artigo País de afiliação: Índia Instituição/País de afiliação: National JALMA Institute for Leprosy and other Mycobacterial Diseases (ICMR)/IN