Short interfering RNAs targeting a vampire-bat related rabies virus phosphoprotein mRNA
Braz. j. microbiol
;
48(3): 566-569, July-Sept. 2017. tab, graf
Artigo
em Inglês
| LILACS
| ID: biblio-889146
ABSTRACT
Abstract The aim of this study was to assess the in vitro and in vivo effects of short-interfering RNAs (siRNAs) against rabies virus phosphoprotein (P) mRNA in a post-infection treatment for rabies as an extension of a previous report (Braz J Microbiol. 2013 Nov 15;44(3)879-82). To this end, rabies virus strain RABV-4005 (related to the Desmodus rotundus vampire bat) were used to inoculate BHK-21 cells and mice, and the transfection with each of the siRNAs was made with Lipofectamine-2000™. In vitro results showed that siRNA 360 was able to inhibit the replication of strain RABV-4005 with a 1 log decrease in virus titter and 5.16-fold reduction in P mRNA, 24 h post-inoculation when compared to non-treated cells. In vivo, siRNA 360 was able to induce partial protection, but with no significant difference when compared to non-treated mice. These results indicate that, despite the need for improvement for in vivo applications, P mRNA might be a target for an RNAi-based treatment for rabies.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Fosfoproteínas
/
Raiva
/
Vírus da Raiva
/
Proteínas Virais
/
Quirópteros
/
RNA Interferente Pequeno
/
Interferência de RNA
Limite:
Animais
Idioma:
Inglês
Revista:
Braz. j. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2017
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Brasil
Instituição/País de afiliação:
University of São Paulo/BR
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