A plate method for rapid screening of Ketogulonicigenium vulgare mutants for enhanced 2-keto-l-gulonic acid production
Braz. j. microbiol
;
48(3): 397-402, July-Sept. 2017. tab, graf
Artigo
em Inglês
| LILACS
| ID: biblio-889149
ABSTRACT
Abstract A new plate method was developed for rapid screening of Ketogulonicigenium vulgare mutants overproducing 2-keto-l-gulonic acid (2-KLG). The screening methodology took the advantage of the acidity caused by 2-KLG, which changes the color of bromothymol blue (pH indicator) from blue to yellow. Using the proposed method, a mutant, K. vulgare 65, was selected from 20,000 colonies produced by a strain subjected to spaceflight mutagenesis. When co-cultured with Bacillus megaterium 2980 in 20-L fermenters, K. vulgare 65 showed a high conversion rate (94.45%) of l-sorbose to 2-KLG. In contrast to the traditional screening method, this one significantly improved the frequency of obtaining positive mutants. The proposed plate screening method is cost-effective and easy to run and is thus useful for the isolation and screening of K. vulgare mutants overproducing 2-KLG.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Açúcares Ácidos
/
Técnicas Bacteriológicas
/
Rhodobacteraceae
Tipo de estudo:
Estudo diagnóstico
/
Estudos de avaliação
/
Estudo de rastreamento
Idioma:
Inglês
Revista:
Braz. j. microbiol
Assunto da revista:
Microbiologia
Ano de publicação:
2017
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
China
Instituição/País de afiliação:
Institute of Applied Ecology/CN
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