Development and validation of stability indicating liquid chromatographic (RP-HPLC) method for estimation of ubidecarenone in bulk drug and formulations using quality by design (QBD) approach
Braz. J. Pharm. Sci. (Online)
;
53(4): e17293, 2017. tab, graf, ilus
Artigo
em Inglês
| LILACS
| ID: biblio-889435
ABSTRACT
ABSTRACT A novel, accurate, precise and economical stability indicating Reverse Phase-High Performance Liquid Chromatography (RP-HPLC) method, was developed and validated for the quantitative determination of ubidecarenone (UDC) in bulk drug, UDC marketed formulation and UDC loaded cubosomes (CBMs) nanocarriers through Response surface methodology (RSM) design with three factors and three levels was performed to optimize the chromatographic variables followed by forced degradation studies of UDC were performed to detect degradation peak. RP-HPLC separation was achieved using mobile phase consisting of AcetonitrileTetrahydrofuranDeionised water in the ratio 55423 and a flow rate of 1.0 mL/min was optimized with a standard retention time (Rt) of 2.15 min, through experiment. The method was found linear in the concentration range of 5-100 µg/mL with a regression coefficient of 0.999. The limit of detection (LOD) and limit of quantification (LOQ) were found to be 3.04 µg/mL and 9.11 µg/mL, respectively.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Ubiquinona
/
Cromatografia de Fase Reversa
Idioma:
Inglês
Revista:
Braz. J. Pharm. Sci. (Online)
Assunto da revista:
Farmacologia
/
Teraputica
/
Toxicologia
Ano de publicação:
2017
Tipo de documento:
Artigo
/
Documento de projeto
País de afiliação:
Índia
/
Arábia Saudita
Instituição/País de afiliação:
Glocal University/IN
/
King Saud University/SA
/
School of Pharmaceutical Education & Research/IN
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