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Do different strains of E. faecalis have the same behavior towards intracanal medications in in vitro research?
Zancan, Rafaela Fernandes; Canali, Lyz Cristina Furquim; Tartari, Talita; Andrade, Flaviana Bombarda de; Vivan, Rodrigo Ricci; Duarte, Marco Antonio Hungaro.
  • Zancan, Rafaela Fernandes; Universidade de São Paulo - USP. Bauru Dental School. Department of Restorative Dentistry. Bauru. BR
  • Canali, Lyz Cristina Furquim; Universidade de São Paulo - USP. Bauru Dental School. Department of Restorative Dentistry. Bauru. BR
  • Tartari, Talita; Universidade de São Paulo - USP. Bauru Dental School. Department of Restorative Dentistry. Bauru. BR
  • Andrade, Flaviana Bombarda de; Universidade de São Paulo - USP. Bauru Dental School. Department of Restorative Dentistry. Bauru. BR
  • Vivan, Rodrigo Ricci; Universidade de São Paulo - USP. Bauru Dental School. Department of Restorative Dentistry. Bauru. BR
  • Duarte, Marco Antonio Hungaro; Universidade de São Paulo - USP. Bauru Dental School. Department of Restorative Dentistry. Bauru. BR
Braz. oral res. (Online) ; 32: e46, 2018. tab, graf
Artigo em Inglês | LILACS | ID: biblio-889469
ABSTRACT
Abstract The aim of this study was to evaluate the antimicrobial action of different endodontic pastes against Enterococcus faecalis ATCC 29212, isolated from the urinary tract, and compare the action with E. faecalis ATCC 4083, isolated from the root canal. For this purpose, dentin blocks were infected for 21 days with both bacteria at different time-intervals to ensure there would be no cross contamination. After this period, blocks were immersed in the test medications for 7 days, according to the following groups CH/S, CH/P, CH/CMCP, CH/CHX, CH/DAP and TAP. Images of the samples were captured with a confocal microscope and the percentage of live cells was computed by means of the Bioimage program. The ATCC 29212 strain was shown to be more resistant to CH/SS, Calen, CH/DAP, and TAP than the ATCC 4083 strain. The antimicrobial action of the medications against each strain were divergent concerning the order of susceptibility. The authors concluded that the strains behaved in a different manner in general, those extracted from the urinary tract were more resistant to the tested medications. Therefore, when E. faecalis must be used for in vitro research in endodontics, we suggest the use of ATCC 4083 strain to obtain results that are closer to the clinical reality.
Assuntos


Texto completo: DisponíveL Índice: LILACS (Américas) Assunto principal: Hidróxido de Cálcio / Clorexidina / Enterococcus faecalis / Biofilmes / Cavidade Pulpar / Antibacterianos Limite: Animais Idioma: Inglês Revista: Braz. oral res. (Online) Assunto da revista: Odontologia Ano de publicação: 2018 Tipo de documento: Artigo / Documento de projeto País de afiliação: Brasil Instituição/País de afiliação: Universidade de São Paulo - USP/BR

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Texto completo: DisponíveL Índice: LILACS (Américas) Assunto principal: Hidróxido de Cálcio / Clorexidina / Enterococcus faecalis / Biofilmes / Cavidade Pulpar / Antibacterianos Limite: Animais Idioma: Inglês Revista: Braz. oral res. (Online) Assunto da revista: Odontologia Ano de publicação: 2018 Tipo de documento: Artigo / Documento de projeto País de afiliação: Brasil Instituição/País de afiliação: Universidade de São Paulo - USP/BR