miR-483-5p promotes prostate cancer cell proliferation and invasion by targeting RBM5
Int. braz. j. urol
;
43(6): 1060-1067, Nov.-Dec. 2017. graf
Artigo
em Inglês
| LILACS
| ID: biblio-892928
ABSTRACT
ABSTRACT Objective:
miR-483-5p has been identified as a miRNA oncogene in certain cancers. However, its role in prostate cancer has not been sufficiently investigated. In this study, we investigated the role of miR-483-5p in prostate cancer and examined RBM5 regulation by miR-483-5p. Material andmethods:
Expression levels of miR-483-5p were determined by quantitative real-time PCR. The effect of miR-483-5p on proliferation was evaluated by MTT assay, cell invasion was evaluated by trans-well invasion assays, and target protein expression was determined by western blotting in LNCaP, DU-145, and PC-3 cells. Luciferase reporter plasmids were constructed to confirm the action of miR-483-5p on downstream target gene RBM5 in HEK-293T cells.Results:
we observed that miR-483-5p was upregulated in prostate cancer cell lines and tissues. A miR-483-5p inhibitor inhibited prostate cancer cell growth and invasion in DU-145 and PC-3 cells. miR-483-5p directly bound to the 3' untranslated region (3'UTR) of RBM5 in HEK-293T cells. RBM5 overexpression inhibited prostate cancer cell growth and invasion in LNCaP cells. Enforced RBM5 expression alleviated miR-483-5p promotion of prostate cancer cell growth and invasion in LNCaP cells.Conclusion:
The present study describes a potential mechanism underlying a miR-483-5p/RBM5 link that contributes to prostate cancer development.
Texto completo:
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Índice:
LILACS (Américas)
Assunto principal:
Neoplasias da Próstata
/
Regulação Neoplásica da Expressão Gênica
/
Proteínas de Ciclo Celular
/
Regiões não Traduzidas
/
Proteínas Supressoras de Tumor
/
MicroRNAs
/
Proliferação de Células
/
Proteínas de Ligação a DNA
/
Reação em Cadeia da Polimerase em Tempo Real
Limite:
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Int. braz. j. urol
Assunto da revista:
Urologia
Ano de publicação:
2017
Tipo de documento:
Artigo
País de afiliação:
China
Instituição/País de afiliação:
Inner Mongolia Medical University/CN
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