RUNX2 and WWOX genes as molecular biomarkers and candidates for targeted therapy in Egyptian patients with primary conventional osteosarcoma
Appl. cancer res
;
36: 1-9, 2016. tab, ilus
Artigo
em Inglês
| LILACS, Inca
| ID: biblio-910946
ABSTRACT
Background:
The conventional osteosarcoma (OS) is the commonest primary malignant, bone tumor with complex genomic profiles and poor survival. Runt-related transcription factor 2 (RUNX2) and WW domain containing oxidoreductase (WWOX) genes are implicated in normal osteogenesis as well as in the development of primary conventional OS.Methods:
We retrospectively assessed protein and RNA expression of the RUNX2 and WWOX genes by quantitative real time PCR (qPCR) and immunohistochemistry (IHC) in 80 cases of primary OS and 20 normal control (NC) subjects. Proteins and RNA expression levels of both genes were correlated to clinico-pathological features of the patients, progression free and overall survival (PFS& OS) rates.Results:
In OS, RUNX2 protein was detected in 72/80 (90%) cases compared to 4/20 (20%) NC samples (p. < 0.001) and RUNX2-RNA was up regulated (up to 103.2 folds) in 60/80 (75%) (p = 0.01). WWOX protein and RNA (up to 7.2 folds) were detected in all NC samples but in 24/80 (30%) and 20/80 (20%) OS cases; respectively (p. < 0.001 for each). The concordance between the RNA and protein expressions for RUNX2 and WWOX was significantly high (X_trend
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
RNA
/
Biomarcadores Tumorais
/
Osteossarcoma
/
Diferenciação Celular
/
Estudos Retrospectivos
/
Subunidade alfa 1 de Fator de Ligação ao Core
/
Terapia de Alvo Molecular
/
Oxidorredutase com Domínios WW
Tipo de estudo:
Estudo observacional
/
Estudo prognóstico
Limite:
Adulto
/
Feminino
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Appl. cancer res
Assunto da revista:
Neoplasias
Ano de publicação:
2016
Tipo de documento:
Artigo
País de afiliação:
Egito
Instituição/País de afiliação:
Alexandria University/EG
/
National Cancer Institute, Cairo University/EG
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