Cirrhosis in rats does not resolve in the long-term after induction by thioacetamide model
Braz. j. morphol. sci
;
31(1): 33-41, 1/3/2014. ilus, tab
Artigo
em Inglês
| LILACS
| ID: biblio-912339
ABSTRACT
Introductions Hepatic cirrhosis is a final common pathway of all chronic liver diseases, characterized by deposit of fibrillar collagen and liver failure. Materials and Methods:
In this experiment, hepatic cirrhosis was induced in 15 female Wistar rats by a 14-week period, with thioacetamide solution in a 200 mg/kg dosage, via intraperitoneal. Animals were submitted to liver biopsy, and euthanized after a 80-day post-induction period. Serum biochemical analysis was performed, in addition to histopathology by H.E., Picrosirius, Alcian Blue and P.A.S. stainings, following analysis of histological activity index and staging of fibrosis. Morphometric analysis of collagen on Picrosirius slides was also performed.Results:
Mortality during experimental period was low (13.33%), and after 80-day period, liver function improved, cellular changes did not altered, and deposition of acidic mucopolysaccharides and glycogen were increased. Liver histological activity did not change significantly (7.25 ± 1.30 to 6.41 ± 1.32), but staging of fibrosis was altered (3.91 ± 0.76 to 4.70 ± 1.11). Interlobular collagen showed a significant decrease (5.14 ± 2.00 to 4.00 ± 1.20), while intralobular collagen was increased (0.23 ± 0.06 to 0.36 ± 0.08).Conclusions:
These findings characterize thioacetamide as a safe experimental model for induction cirrhosis, which may be used for future therapy studies.(AU)
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Tioacetamida
/
Colágeno
/
Modelos Animais de Doenças
/
Cirrose Hepática
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
Idioma:
Inglês
Revista:
Braz. j. morphol. sci
Assunto da revista:
Anatomia
Ano de publicação:
2014
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
University of São Paulo/BR
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