Protein microarray for complex apoptosis monitoring of dysplastic oral keratinocytes in experimental photodynamic therapy
Biol. Res
;
47: 1-9, 2014. ilus, graf
Artigo
em Inglês
| LILACS
| ID: biblio-950729
ABSTRACT
BACKGROUND:
Photodynamic therapy is an alternative treatment of muco-cutaneous tumors that uses a light source able to photoactivate a chemical compound that acts as a photosensitizer. The phthalocyanines append to a wide chemical class that encompasses a large range of compounds; out of them aluminium-substituted disulphonated phthalocyanine possesses a good photosensitizing potential.RESULTS:
The destructive effects of PDT with aluminium-substituted disulphonated phthalocyanine are achieved by induction of apoptosis in tumoral cells as assessed by flow cytometry analysis. Using protein microarray we evaluate the possible molecular pathways by which photodynamic therapy activates apoptosis in dysplastic oral keratinocytes cells, leading to the tumoral cells destruction. Among assessed analytes, Bcl-2, P70S6K kinase, Raf-1 and Bad proteins represent the apoptosis related biomolecules that showed expression variations with the greatest amplitude.CONCLUSIONS:
Up to date, the intimate molecular apoptotic mechanisms activated by photodynamic therapy with this type of phthalocyanine in dysplastic human oral keratinocytes are not completely elucidated. With protein microarray as high-throughput proteomic approach a better understanding of the manner in which photodynamic therapy leads to tumoral cell destruction can be obtained, by depicting apoptotic molecules that can be potentially triggered in future anti-tumoral therapies.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Fotoquimioterapia
/
Lesões Pré-Cancerosas
/
Neoplasias Bucais
/
Queratinócitos
/
Apoptose
/
Análise Serial de Proteínas
Limite:
Humanos
Idioma:
Inglês
Revista:
Biol. Res
Assunto da revista:
Biologia
Ano de publicação:
2014
Tipo de documento:
Artigo
País de afiliação:
Romênia
Instituição/País de afiliação:
National Institute for Research & Development in Chemistry and Petrochemistry/RO
/
National Institute of Pathology/RO
/
University of Medicine and Pharmacy/RO
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