Anti-inflammatory components of the Vietnamese starfish Protoreaster nodosus
Biol. Res
;
48: 1-9, 2015. ilus, graf, tab
Artigo
em Inglês
| LILACS
| ID: biblio-950776
ABSTRACT
BACKGROUND: In the present study, we examined the inhibitory effects of a methanolic extract, dichloromethane fraction, water layer, and polyhydroxylated sterols (1-4) isolated from the Vietnamese starfish Protoreaster nodosus on pro-inflammatory cytokine (IL-12 p40, IL-6, and TNF-α) production in LPS-stimulated bone marrow-derived dendritic cells (BMDCs) using enzyme-linked immunosorbent assays (ELISA). RESULTS: The methanolic extract and dichloromethane fraction exerted potent inhibitory effects on the production of all three pro-inflammatory cytokines, with IC50 values ranging from 0.60 ± 0.01 to 26.19 ± 0.64 µg/mL. Four highly pure steroid derivatives (1-4) were isolated from the dichloromethane fraction and water layer of P. nodosus. Potent inhibitory activities were also observed for (25S)5α-cholestane-3ß,4ß,6α,7α,8ß,15α,16ß,26-octol (3) on the production of IL-12 p40 and IL-6 (IC50s = 3.11 ± 0.08 and 1.35 ± 0.03 µM), and for (25S) 5α-cholestane-3ß,6α,8ß,15α,16ß,26-hexol (1) and (25S)5α-cholestane-3ß,6α,7α,8ß,15α,16ß,26-heptol (2) on the production of IL-12 p40 (IC50s = 0.01 ± 0.00 and and 1.02 ± 0.01 µM). Moreover, nodososide (4) exhibited moderate inhibitory effects on IL-12 p40 and IL-6 production. CONCLUSION: This is the first report of the anti-inflammatory activity from the starfish P. nodosus. The main finding of this study is the identification oxygenated steroid derivatives from P. nodosus with potent anti-inflammatory activities that may be developed as therapeutic agents for inflammatory diseases.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Estrelas-do-Mar
/
Células Dendríticas
/
Interleucina-6
/
Fator de Necrose Tumoral alfa
/
Subunidade p40 da Interleucina-12
/
Anti-Inflamatórios
Tipo de estudo:
Estudo prognóstico
Limite:
Animais
País/Região como assunto:
Ásia
Idioma:
Inglês
Revista:
Biol. Res
Assunto da revista:
Biologia
Ano de publicação:
2015
Tipo de documento:
Artigo
País de afiliação:
Índia
/
Coréia do Sul
/
Vietnã
Instituição/País de afiliação:
Chungnam National University/KR
/
Jeju National University/KR
/
Vietnam Academy of Science and Technology/VN
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