Decreased VEGF-A and sustained PEDF expression in a human retinal pigment epithelium cell line cultured under hypothermia
Biol. Res
; 48: 1-8, 2015. ilus, graf, tab
Article
em En
| LILACS
| ID: biblio-950806
Biblioteca responsável:
CL1.1
ABSTRACT
BACKGROUND:
Previous reports have described a decrease in retinal temperature and clinical improvement of wet age-related macular degeneration (AMD) after vitrectomy. We hypothesized that the retinal temperature decrease after vitrectomy plays a part in the suppression of wet AMD development. To test this hypothesis, we evaluated the temperature dependence of the expression of vascular endothelial growth factor-A (VEGF-A) and in vitro angiogen-esis in retinal pigment epithelium (RPE).RESULTS:
We cultured ARPE-19 cells at 37, 35, 33 and 31°C and measured the expression of VEGF-A, VEGF-A splicing variants, and pigment epithelium-derived factor (PEDF). We performed an in vitro tube formation assay. The dehydrogenase activity was also evaluated at each temperature. Expression of VEGF-A significantly decreased with decreased temperature while PEDF expression did not. VEGF165 expression and in vitro angiogenesis also were temperature dependent. The dehydrogenase activity significantly decreased as the culture temperature decreased.CONCLUSIONS:
RPE cultured under hypothermia that decreased cellular metabolism also had decreased VEGF-A and sustained PEDF expression, creating an anti-angiogenic environment. This mechanism may be associated with a beneficial effect after vitrectomy in patients with wet AMD.Palavras-chave
Texto completo:
1
Índice:
LILACS
Assunto principal:
Serpinas
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Fator A de Crescimento do Endotélio Vascular
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Proteínas do Olho
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Epitélio Pigmentado da Retina
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Hipotermia
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Fatores de Crescimento Neural
Limite:
Humans
Idioma:
En
Revista:
Biol. Res
Assunto da revista:
BIOLOGIA
Ano de publicação:
2015
Tipo de documento:
Article