CMPD1 inhibited human gastric cancer cell proliferation by inducing apoptosis and G2/M cell cycle arrest
Biol. Res
; 51: 11, 2018. graf
Article
em En
| LILACS
| ID: biblio-950897
Biblioteca responsável:
CL1.1
ABSTRACT
BACKGROUND:
Gastric cancer occupies the fourth highest morbidity rate of cancers worldwide. Clinical therapies of gastric cancer remain limited because of uncertainty of mechanisms and shortness of effective medicine. Thus, new drug candidates for gastric cancer treatment is urgently needed.RESULTS:
In this study, CMPD1 as a wildly used MK2 phosphorylation inhibitor was employed to find its impact on gastric cancer cell proliferation, apoptosis and cell cycle using colony formation assay and flow cytometry analysis. Along with its anti-proliferation effect on gastric cancer cell line MKN-45 and SGC7901, CMPD1 also induced massive apoptosis and significant G2/M phase arrest in a time-dependent and dose-dependent manner in MKN-45 cells respectively. Furthermore, Western blot confirmed that the expression of anti-apoptotic proteins Bcl-2 was decreased while BAX, cytochrome c release and cleaved PARP were increased. In addition, oncogene c-Myc was downregulated in response to CMPD1 treatment.CONCLUSIONS:
Our results demonstrated that CMPD1 has anti-tumor effect on human gastric cancer cell line MKN- 45 possibly via downregulating oncogene c-Myc expression and CMPD1 could be applied as a potential candidate for treating gastric malignancy. To the best of our knowledge, it is the first report of anti-tumor effect of CMPD-1 on human gastric cancer cells.Palavras-chave
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Índice:
LILACS
Assunto principal:
Neoplasias Gástricas
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Proteínas Serina-Treonina Quinases
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Apoptose
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Peptídeos e Proteínas de Sinalização Intracelular
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Proliferação de Células
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Fatores de Transcrição SOX9
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Pontos de Checagem da Fase G2 do Ciclo Celular
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Antineoplásicos
Tipo de estudo:
Evaluation_studies
Limite:
Humans
Idioma:
En
Revista:
Biol. Res
Assunto da revista:
BIOLOGIA
Ano de publicação:
2018
Tipo de documento:
Article