Effects of IL-10 and glucose on expression of OPG and RANKL in human periodontal ligament fibroblasts
Braz. j. med. biol. res
;
49(4): e4324, 2016. tab, graf
Artigo
em Inglês
| LILACS
| ID: biblio-951663
ABSTRACT
The effects of interleukin-10 (IL-10) and glucose on mRNA and protein expression of osteoprotegerin (OPG), and its ligand, receptor activator of nuclear factor-κB ligand (RANKL), were investigated in human periodontal ligament fibroblasts (HPDLFs). Primary HPDLFs were treated with different concentrations of IL-10 (0, 1, 10, 25, 50, and 100 ng/mL) or glucose (0, 5.5, 10, 20, 30, and 40 mmol/L). Changes in mRNA and protein expression were examined using the reverse-transcription polymerase chain reaction (RT-PCR) and Western blot analysis, respectively. After IL-10 treatment, mRNA and protein levels of OPG were increased, while mRNA and protein levels of RANKL were decreased (P<0.05), both in a concentration-dependent manner. Glucose stimulation had the opposite concentration-dependent effect to that of IL-10 on OPG and RANKL expression. IL-10 upregulated OPG expression and downregulated RANKL expression, whereas high glucose upregulated RANKL and downregulated OPG in HDPLFs. Abnormal levels of IL-10 and glucose may contribute to the pathogenesis of periodontal disease.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Ligamento Periodontal
/
Interleucina-10
/
Ligante RANK
/
Osteoprotegerina
/
Fibroblastos
/
Glucose
Limite:
Humanos
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
2016
Tipo de documento:
Artigo
País de afiliação:
China
Instituição/País de afiliação:
Nanjing Medical University/CN
/
Xi'an Jiaotong University College of Medicine/CN
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