Pre-analytical parameters associated with unsuccessful karyotyping in myeloid neoplasm: a study of 421 samples
Braz. j. med. biol. res
;
52(2): e8194, 2019.
Artigo
em Inglês
| LILACS
| ID: biblio-984032
ABSTRACT
Cytogenetics is essential in myeloid neoplasms (MN) and pre-analytical variables are important for karyotyping. We assessed the relationship between pre-analytical variables (time from collection to sample processing, material type, sample cellularity, and diagnosis) and failures of karyotyping. Bone marrow (BM, n=352) and peripheral blood (PB, n=69) samples were analyzed from acute myeloid leukemia (n=113), myelodysplastic syndromes (n=73), myelodysplastic syndromes/myeloproliferative neoplasms (n=17), myeloproliferative neoplasms (n=137), and other with conclusive diagnosis (n=6), and reactive disorders/no conclusive diagnosis (n=75). The rate of unsuccessful karyotyping was 18.5% and was associated with the use of PB and a low number of nucleated cells (≤7×103/µL) in the sample. High and low cellularity in BM and high and low cellularity in PB samples showed no metaphases in 3.9, 39.7, 41.9, and 84.6% of cases, respectively. Collecting a good BM sample is the key for the success of karyotyping in MN and avoids the use of expensive molecular techniques.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Manejo de Espécimes
/
Síndromes Mielodisplásicas
/
Células da Medula Óssea
/
Leucemia Mieloide
/
Cariotipagem
/
Transtornos Mieloproliferativos
Tipo de estudo:
Estudo diagnóstico
/
Fatores de risco
Limite:
Adolescente
/
Adulto
/
Idoso
/
Aged80
/
Feminino
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
Braz. j. med. biol. res
Assunto da revista:
Biologia
/
Medicina
Ano de publicação:
2019
Tipo de documento:
Artigo
País de afiliação:
Brasil
Instituição/País de afiliação:
Hospital Israelita Albert Einstein/BR
/
Monte Tabor+BR
/
Hospital de Câncer de Mato Grosso/BR
/
Universidade Federal do Ceará/BR
Similares
MEDLINE
...
LILACS
LIS