Expression of M30 and M65 in celiac disease. Analytical cross-sectional study
São Paulo med. j
;
136(6): 525-532, Nov.-Dec. 2018. tab, graf
Artigo
em Inglês
| LILACS
| ID: biblio-991701
ABSTRACT
ABSTRACT BACKGROUND:
The role of villous atrophy in apoptosis, a distinctive feature of celiac disease, is a matter of controversy. The aim of this study was to determine the apoptosis rate through immunohistochemical staining for M30 and M65 in celiac disease cases. DESIGN ANDSETTING:
Analytical cross-sectional study in a tertiary-level center.METHODS:
Duodenal biopsies from 28 treatment-naive patients with celiac disease, 16 patients with potential celiac disease, 10 patients with a gluten-free diet and 8 controls were subjected to immunohistochemical staining for the end-apoptotic marker M30 and the total cell death marker M65. H-scores were compared. Several laboratory parameters were recorded concomitantly, and at the one-year follow-up for celiac disease and potential celiac disease patients.RESULTS:
There was a significant difference in H-score for M30 expression between the celiac disease, potential celiac disease and gluten-free diet groups (P = 0.009). There was no significant difference in H-score for M65 expression. There was a positive correlation between the H-score for M30 expression and the anti-tissue transglutaminase immunoglobulin A (anti-tTgIgA) and anti-tissue transglutaminase immunoglobulin G (anti-tTgIgG) levels (R = 0.285, P = 0.036; and R = 0.307, P = 0.024, respectively); and between the H-score for M65 expression and the anti-tTgIgA and anti-tTgIgG levels (R = 0.265, P = 0.053; and R=0.314, P = 0.021, respectively). There was no difference between celiac disease and potential celiac disease patients regarding the laboratory parameters selected.CONCLUSION:
The rates of apoptosis and nutritional deficiencies in patients with potential celiac disease were similar to those in patients with celiac disease.
Texto completo:
DisponíveL
Índice:
LILACS (Américas)
Assunto principal:
Doença Celíaca
/
Apoptose
/
Caspases
/
Queratina-18
Tipo de estudo:
Estudo observacional
/
Estudo de prevalência
/
Fatores de risco
Limite:
Adulto
/
Feminino
/
Humanos
/
Masculino
Idioma:
Inglês
Revista:
São Paulo med. j
Assunto da revista:
Cirurgia Geral
/
Cincia
/
Ginecologia
/
Medicina
/
Medicina Interna
/
Obstetr¡cia
/
Pediatria
/
Sa£de Mental
/
Sa£de P£blica
Ano de publicação:
2018
Tipo de documento:
Artigo
País de afiliação:
Turquia
Instituição/País de afiliação:
Ankara University Faculty of Medicine/TR
/
Keçiören Training and Research Hospital/TR
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