[Determination of chromosomal changes in DMBA-induced skin cancer in SD rat strains]

ABSTRACT

Skin cancer is one of the most important cancers in the world. This cancer is more common in men than women. We survey chromosomal changes in DMBA-induced skin cancer in SD rat strains. In this fundamental study, 20 SD rat strains were randomly divided into case and contal group. DMBA [2.5 mg] was injected to SD rat strains subcutaneously; therefore skin cancer model for studies was created. Tumors became subjects for cell culture and metaphase chromosomal were prepared. Finally g-banding were stained. We have also transmitted genomic information from rat to human using suitable databases and Gene were determined. Data showed numerical and frequent structural changes in different number of chromosomes. For example; gain in chromosomes number 1, 15, 17 and loss in 1, 7, 15, and also structural changes like deletion was seen in chromosomes number 1, 4, 8, 10, 15, 17, and addition in chromosome number 15. it is predicted that CST6, PRKCDBP, PTCH1, DKK3, BRMS1, CDKN1C, CD81, DMP1, CDKN2B, EEF1A1, HRAS, CASP2, KLF4 probably cause skin cancer