Role of P27 [Kip 1] and MUC1 in papillary transitional cell carcinoma of the urinary bladder

ABSTRACT

To examine P27 and MUC1 protein expression in specimens of papillary transitional cell carcinoma-urinary bladder and to compare both of them with the tumor outcome. Paraffin-sections from previously diagnosed papillary transitional cell carcinoma-urinary bladder were graded, staged and followed up for 5 years. Ten non neoplastic urologicl lesions diagnosed as polypoid cystitis were taken as a control. Three sections of 4 micron thickness were obtained for every case. One is H and E stained for diagnosis reviewing and confirmation. The other 2 sections are immunohistochemically stained for both P27 and MUC1. The data of immunohistochemical results were correlated with the following conventional prognostic variables tumor grade, stage, distant metastasis and 5-year survival of papillary transitional cell carcinoma [PTCC]-urinary bladder. The results showed a highly significant and an insignificant relationships between P27 expression and tumor grade and stage respectively [p<0.01 and >0.05]. Correlating P27 expression with distant metastasis and overall survival showed a significant relationship with distant metastasis [p<0.05] and a highly significant one with overall survival [p<0.01].The results showed also a significant relationships between MUC1 expression and both tumor grade and 5-year overall survival [p<0.05 for both]. In conclusion, P27 and MUC1 immunohistochemistry may augment the classic histochemistry for prognosis of PTCC as well as prediction of the patient outcome. Specific oncogene markers are more significant than oncogene phenotype markers in clinical diagnosis and screening of PTCC