[Evaluation of IL-1 beta secretion level by human osteoblast cells adjucent to white MTA, dark MTA, portland cement and IRM as a retrograde filling material]

ABSTRACT

Osteoblasts and ligament periodontal cells are the essential cells for wound repair after root-end resections and perforation repair. Osteoblast cells reaction in direct contact with filling materials play a critical role in wound repair after surgeries and perforation corrections. Cell attachment on material surfaces and cell secretory function is the primary phase for evaluation of normal cell function. The aim of this study was to morphologically evaluate osteoblast cells [MG-63] function and IL-1 beta secretory level adjacent to gray MTA, white MTA, Portland cement and IRM, as materials for root-end fillings and repairing perforations. Human osteoblast cells [MG-63] obtained from Iran Pasteur Institute. cell bank were grown in RPMI-1640 medium. The under study materials, following the company instructions, were mixed and seeded in 24, 1cmx1cm partition plates with the aproximate thickness of 1mm. The cells were added after the materials primary setting time. The cells were observed by a light microscope an day 1, 3 and 7. In the mentioned intervals, the cells supernants were collected and examined by ELISA and the amount of IL-1 beta in each specimen was measured in pg/ml. The turkey test was used for comparison of the data among different matreials and the Komogrov-Smirnov test was used for normalizing the resonses. The cells morphological outcomes illustrated that after 7 days, a large amount of osteoblasts adjacent to gray and white MTA had the good attachment and morpholigically expansion and flat. The cells adjacent to Portland cement were found round and mostly seperated from the surface, although some flat cells could be found among them. Adjacent to IRM, all the cells were round and seperated from the plate surface. The level amount of IL-1 beta secretion adjacent to gray and white MTA was significantly more than to IRM and Portland cement [P=0.00]. Adjacent to gray and white MTA, the amount of IL-1 beta secretion was not significantly different [P=0.77], also the IL-1 beta secretion level adjacent to Portland cement and IRM was not significantly different [P=0.187]. The currrent study result indicates that human osteoblasts adjacent to gray MTA and white MTA, in comparison to Portland cement and IRM, showed a more appropriate response. Therefore we recommend the use of MTA over the other materials. Regarding Portland cement more research needs to be done in order to reach a final conclusion: