T2-toxin hepatotoxicity in the in situ rat liver model
IJPR-Iranian Journal of Pharmaceutical Research. 2004; 3 (4): 225-230
em Inglês
| IMEMR
| ID: emr-102852
ABSTRACT
T-2 toxin, a trichothecene mycotoxin, is considered to be one of the most toxic compounds that are produced by molds, particularly the Fusarium species. Fusarium species have been recognized as a great agricultural problem. They occur worldwide on a variety of plant hosts and cereal grains. The aim of this study was to investigate T-2 toxin-induced liver injury using in situ perfused rat liver. The in situ perfused rat liver [IPRL] was chosen because it permits studies of liver function in a system that resembles normal physiology. Elevation of aminotransferase activities have shown to be a good indicator of hepatocellular damage. In addition, glutathione levels have also shown to be an indicator of liver damage through lipid peroxidation. Male Sprague-Dawley rats [6-8 weeks] weighing 250-300 g were used in this study. They were randomly divided into 5 groups of 3-4 rats per cage. In group 1, liver was perfused by Krebs-Henseleit buffer alone [Control]. Groups 2-5 received different concentration of T-2 toxin [4, 9, 21, 43 [rho]mol/L] in Krebs-Henseleit buffer and biochemical changes in the liver were examined within 2 h. There was a significant increase in both ALT and AST activity in all dose levels compared with the control group [p<0.01 and p<0.05]. T-2 toxin treatment enhanced lipid peroxidation in the liver, as indicated by the increased MDA content in liver homogenates. The MDA level was maximal 2 h after the T-2 toxin challenge [p<0.01 and p<0.05]. The results also show that T-2 toxin causes an increase in lipid peroxidation while causing a decrease in glutathione [GSH] content in bile secretion [p<0.01]. This result suggests that both lipid peroxidation and glutathione [GSH] depletion play a role in T-2 toxin liver induced damages
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Índice:
IMEMR (Mediterrâneo Oriental)
Assunto principal:
Doenças das Plantas
/
Peroxidação de Lipídeos
/
Ratos Sprague-Dawley
/
Modelos Animais
/
Fusarium
/
Glutationa
/
Fígado
/
Testes de Função Hepática
/
Micotoxinas
Limite:
Animais
Idioma:
Inglês
Revista:
Iran. J. Pharm. Res.
Ano de publicação:
2004
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