In vitro culture of Helicobacter pylori: comparative analysis of different media and sensitivity testing

ABSTRACT

100 gastric biopsies, in two transport media, were homogenized and cultured on three media under microaerophilic conditions. Columbia agar with commercial antibiotic supplement [containing 10 mg Vancomycin, 05mg trimethoprim, 05 mg cefsulodin and 05 mg ampotericin B per litre] yielded the best results. Growth was obtained in 3-7 days. The resistance to nalidixic acid and rapid urease production of H. pylon could be used to differentiate this species form cainpylobacter spp. Metronidazole resistance was found in 27/43[63%] of isolates. All the isolates were found to be sensitive to Amoxycillin, Clarithromycin and Tetracycline