[Prevelancey MTHFR C677T polymorphism as a genetic risk factor for cardiovascular disease in the normal Iranian population]

ABSTRACT

Two hundred and eight asymptomatic individuals from different origins of Iran were included in this study in order to assess the distribution of the MTHFR C677T polymorphism responding for cardiovascular disease [CVD] in the general Iranian population using a novel technique reverse hybridization Strip Assay for the rapid and simultaneous detection. The test is based on multiplex PCR and hybridization to a tests trip presenting a parallel array of allele-specific oligonucleotide probes for each mutation. The allele frequencies of mutant MTHFR C677T [0.24] in our cohort were quite comparable with those reported by Golbahar et al. [2005] in Iran. Mutant MTHFR C677T in Iran occurred less frequently than among Europeans, but exceeded the much lower frequencies known from India and most of Asia. Here we describe the distribution of mutant allele MTHFR C677T in different ethnicities of Iranian population and compare the results to previously reported data. Our data represent the most comprehensive study to date with respect to thrombophilic gene polymorphism in Iran