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[Molecular epidemiology of foot-and-mouth disease virus in ruminant during 2005-2006 in Iran]
Journal of Veterinary Research. 2010; 65 (2): 123-128
em Persa | IMEMR | ID: emr-105422
ABSTRACT
Foot and mouth disease [FMD] is a highly contagious viral disease of ruminant which causes fever and postule on mouth, hoof and teat. The main purpose of this study was to determine and charachtrize isolated FMD virus from Iran between 2005-2006, and to compare it with vaccine virus strains. After preparation of samples, serological test for typing of virus was performed. In order to virus isolation. the samples were inoculated to IBRS2 cell, RT-PCR and PCR were used for sequencing. Two dimentional virus neutralization test was carried out for detecting of immunological relationship [r value] between the field isolate and virus presented in vaccine. Detected strains were as follows 241 samples of type AO5IR, 125 of type A87IR, 79 of type 0.3 of type Asia and 714 negative out of 1162 samples. Average r-values of type A, 0, Asia field virus with vaccine strains were 50-92% and 97%, respectively. Phylogenic tree was designed according to the nucleic acid sequencing data. There is not strong relationship between field viruses of type A and vaccine viruses. However a strong relationship was shown for type 0 and Asia ones with vaccine virus strains
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Índice: IMEMR (Mediterrâneo Oriental) Assunto principal: Filogenia / Ruminantes / Epidemiologia Molecular / Reação em Cadeia da Polimerase Via Transcriptase Reversa / Vírus da Febre Aftosa Tipo de estudo: Estudo de rastreamento Limite: Animais Idioma: Persa Revista: J. Vet. Res. Ano de publicação: 2010

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Índice: IMEMR (Mediterrâneo Oriental) Assunto principal: Filogenia / Ruminantes / Epidemiologia Molecular / Reação em Cadeia da Polimerase Via Transcriptase Reversa / Vírus da Febre Aftosa Tipo de estudo: Estudo de rastreamento Limite: Animais Idioma: Persa Revista: J. Vet. Res. Ano de publicação: 2010