Detection of molecular markers of toxoplasmosis among Egyptian patients with miscarriage using avidity IGG-ELISA and western blotting
Journal of the Egyptian Society of Parasitology. 2008; 38 (2): 537-546
em Inglês
| IMEMR
| ID: emr-106000
ABSTRACT
A total of 54 miscarriage patients were divided into 3 groups. GI 10 toxoplasmosis patients with +ve IgM-ELISA; GII 24 toxoplasmosis patients with +ve IgG-ELISA, and G III 20 non-toxoplasmosis cross-matched females as a control. All groups were subjected to IgG-avidity ELISA and IgG-avidity immunoblotting. Avidity Indices [AI] by ELISA ranged from 22.6% to 73.3% in GI and from 9.6%-75.6% in GII AI were high [>40%] in 3 [30%] patients in G I and in 8 [33.3%] patients in G II. Sera of GI recognized the 20, 28,32,60,93 and l00 Kda bands with 55% reduction in the 38 and 60 Kda bands after treatment with 6 M urea solutions. Sera of G II recognized the 20, 28, 32, 38, 45, 95-97 and 106 Kda bands. There was 12.5%, 16.6% and 16.7% reduction in the 20, 32, and 106 Kda bands, respectively, after urea. The 38 and 60 Kda bands were identified as good diagnostic markers for the recent toxoplasmosis infection [GI]. The 20, 32 and 106 Kda bands were good markers of high avidity antibodies during the chronic toxoplasmosis [GIl]
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Índice:
IMEMR (Mediterrâneo Oriental)
Assunto principal:
Mulheres
/
Imunoglobulina G
/
Imunoglobulina M
/
Ensaio de Imunoadsorção Enzimática
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Immunoblotting
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Aborto Espontâneo
/
Western Blotting
Tipo de estudo:
Ensaio Clínico Controlado
Limite:
Feminino
/
Humanos
Idioma:
Inglês
Revista:
J. Egypt. Soc. Parasitol.
Ano de publicação:
2008
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