Effect of retinoic acid and peroxisome proliferate on the transfection of alfa-fetoprotein gene in human hepatoma cell lines

ABSTRACT

Retinoic acid [t-RNA] at concentration 1 uM led to deplete CAT gene activity induced by AFP 7300 plasmid transfection in Hep3B [B-virus integrated], whereas in Hu-h7 [non-viral type] the effect was just slight down-regulation of the gene activity. However, in the presence of proxisome proliferator [2 uM] complete absence of CAT reaction was clearly observed in this cell line [Hu-h7]. On the other hand, proxisome proliferator [PP] alone did not show any effect at all in the viral type cell line, [Hep3B], whereas in the non-viral type [Hu- h7], down-regulation of CAT gene function was clearly observed. Therefore, the response of the previous mentioned cell lines [viral and non-viral] either to t-RA or PP was absolutely different. The finding sheds a light on new avenues for differential useful therapy for patient suffering from hepatoma with different past-history of the disease