[Association of T2229/C exon 12 polymorphisms of thyroid peroxidase gene with anti-TPO levels in Tehran population]

ABSTRACT

Antibody secretion in human may be the result of the changes in protein structure. Probably these changes in protein structure or polymorphism in human thyroid peroxidase [TPO] gene is the reason for presence of the anti TPO. In this study, we examined the association of T2229/C exon 12 polymorphism of TPO gene in respect to anti-TPO level. In this study 168 individuals [47 +/- 2 years] were selected as case and control groups based on anti-TPO titer above and below 100 IU/L. PCR-RFLP [polymerase chain reaction-restriction fragment length polymorphism] was used to amplify the segment of exon 12 polymorphism. In exon 12 the allele frequencies were 0.8698 for C allele and 0.1301 for T allele and there was no significant association between this polymorphism and anti-TPO level [CC=127.5 +/- 308 IU/ml vs. TT=126 +/- 224 IU/ml]. This study indicated that there is no significant association between anti-TPO levels and T2229/C exon 12 polymorphisms. Meanwhile, selected SNP of exon12 directly has no effect on anti-TPO levels