Erythrocyte transketolase activity as a function of vitamin B1 after prolonged oral contraceptive administration under different vitamin B1 nutritional status

ABSTRACT

The present investigation is concerned with studying the effect of feeding adult female rats, standard synthetic diet, diets [deprived, deficient and supplemented] with high dose of vitamin B1 on vitamin B1 functional activity during prolonged administration of an oral contraceptive [O.C] [Containing 0.035 mg ethinyl estradiol and 0.5 mg norethindrone]. The experiment lasted for 165 days. Blood samples were withdrawn after 15, 45, 75, 105, 135 and 165 days from O.C administration, for the determination of vitamin B1 level by a functional test, i.e. erythrocyte transketolase [ETK] basal activity and after in-vitro thiamin pyrophosphate addition [TPP]. Results were discussed. It is proved that a proper high dose of vitamin B1 should be given during prolonged oral contraceptive administration