Cardioprotective effect of calcium channel blocers [verapamil] during ischemia/reperfusion after acute haemorrhage

ABSTRACT

An in-vitro study was designed to focus on effects of verapamil on the metabolic potential of cardiac slices after reversible ischemia in rats. The study included two main groups Group A, non ischemia/reperfusion group and group B, ischemia/reperfusion group. Each was subdivided into two subgroups [a and b], each subgroup included ten experiments. Ischemic cardiac slices were obtained from rats subjected to 10 min hemorrhage to induce reversible global ischemia. The results revealed that there was an enhancement in the release of free fatty acids, and lactate and in glucose uptake in group Ba as compared with group Aa. These metabolic alternations produced by ischemic cardiac slices were revealed by verapamil addition but in group Ab, verapamil did not alter the release of FFA and lactate from non- ischemic cardiac slices, whereas it inhibited glucose uptake from these slices. The improvement of the metabolic alternation of ischemic myocardium indicates that verapamil may be of importance in reducing the extent and severity of acute myocardial ischemic injury in acute hemorrhage