[Effect of cyperus rotundus rhizomes on blood glucose, lipid, insulin and hepatic enzymes in insulin resistance model of male rats]

ABSTRACT

Nowadays herbal medicine is excessively used in traditional medicine for diabetes. The Cyperus rotundus rhizomes in traditional medicine are used for decreasing blood glucose and lipid. Therefore, the aim of this study was to examine the effect of rhizomes extract of this plant in insulin resistance model of male rats. This experimental study was carried out on 36 adult male wistar rats in the weight range of 150-200[g]. Diabetes was induced by fructose 10% in drinking water for 8 weeks, then the animals were divided randomly into 4 groups control, Fructose induce, aqueous extracts of Cyperus rotundus rhizomes [500mg/kg], MeOH extracts of Cyperus rotundus rhizomes [500mg/kg]. 48 hours after the last gavage with extracts, the rats were fasted for 12h, then blood samples were collected from heart, serum was dissented and blood glucose, insulin, fasting insulin resistance index [FIRI], lipid profile and hepatic enzymes were determined. Glucose in fructose group in comparison with control group showed a significant increase. Insulin in aqueous and MeOH extracts groups in comparison with control group significantly increased. FIRI in aqueous extract group in comparison with control group was significantly higher [p<0.05]. Lipid profile was not changed in any of them. MeOH extract showed a decrease in all of the hepatic enzymes [Alkaline Phosphatase, Aspartate, and AST, Alanine Transferase]. Aqueous has could not probably be effective in decreasing diabetic complications. But it is believed that with extensive study on MeOH extracts, we can reach a more efficient decrease in diabetes and antihepatotxicity