[Effect of interleukin-12 p40 subunit gene 3'-untranslated region polymorphism in chronic HCV infection]
Scientific Journal of Kurdistan University of Medical Sciences. 2011; 16 (1): 10-19
em Persa
| IMEMR
| ID: emr-110483
ABSTRACT
Production of unusual cytokine levels in hepatitis C infection appears to be associated with progression of the disease, persistence of the virus in host, and establishment of chronic disease. Interleukin-12 as a heterodimeric immunoregulating cytokine is important in the generation of a Th1-based immune response. In this study we investigated the role of IL-12B 3'UTR polymorphism in susceptibility to chronic hepatitis C infection. A total of 126 chronic hepatitis C patients and 136 healthy blood donors were genotyped for IL12-p 40-3' UTR polymorphism. Genotyping was carried out by PCR-RFLP method. The results were confirmed by analyzing 10% of the samples by direct sequencing. We found no significant differences in genotype and allele frequencies of the 3'UTR polymorphism between chronic hepatitis C patients and healthy controls. There was no association between IL12B-3'UTR polymorphism and chronic hepatitis C infection. Our study can be useful in regard to the factors regulating IL-12 production, and its consequent impact on chronic hepatitis C infection susceptibility in Iranian population
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Índice:
IMEMR (Mediterrâneo Oriental)
Assunto principal:
Polimorfismo Genético
/
Polimorfismo de Fragmento de Restrição
/
Reação em Cadeia da Polimerase
/
Regiões 3' não Traduzidas
/
Subunidade p40 da Interleucina-12
/
Técnicas de Genotipagem
Limite:
Humanos
Idioma:
Persa
Revista:
Sci. J. Kurdistan Univ. Med. Sci.
Ano de publicação:
2011
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