effect of blood on apical microleakage of Epiphany and AH26: an in vitro study

ABSTRACT

Blood contamination of the canal during preparation can be a major problem in endodontics; this may result in apical microleakage. The aim of this in vitro study was to evaluate the effect of blood on apical microleakage of a resin-based root canal sealer [AH26] and a polymer-based root canal sealer [Epiphany].In this experimental study, 50 decoronated' central incisors and canine teeth were prepared by RaCe rotary system and randomly divided into 4 groups [n=10]. Groups A[1] and A[2] were obturated by Epiphany/Resilon and AH26/Gutta-percha, respectively. The obturations were performed with a single cone technique after drying root canals. In B[1] and B[2] groups, the test groups, 0.02cc citrated human blood was injected into dried root canals and they were obturated in the same manner. Ten specimens were served as positive and negative controls [n=5].The apical leakage was measured by means of a computerized fluid filtration method after 1 day and 3 weeks. The data was analyzed by One-Sample Kolmogorov-Smirnov, Independent Sample t-test and univariate analysis. Statistical significances were preset at a=0.05. There was no significant difference in apical microleakage of the two sealers after 1 day and 3 weeks in dry and blood environment [P>0.05]. Sealer and environment had no interaction [P>0.05]. Blood contamination has no significant effect on the apical microleakage of Epiphny and AH26