Protective effect of celery oil, vitamin E and their combination against testicular toxicity induced by sodium valporate in male rats

ABSTRACT

The protective effect of celery oil, vitamin E and their combination against testicular toxicity induced by sodium valporate in male rats was studied. Forty nine mature male rats were distributed into 7 equal groups. The 1[st] group was kept as a negative control, while rats of the 2[nd] group were given orally sodium valporate [SVP] in a dose of 500 mg/kg b.wt./day for the last 7 days of the experimental period and used as a positive control. The other protected five groups were pretreated by oral administration of celery oil at two doses [100 and 200 mg/kg b.wt./day] and vitamin E [200 mg/kg b.wt. /day], alone and in combination, for 28 days and received SVP as in the 2[nd] group. At the end of the experiment, the rats were sacrificed and sexual organs were removed and weighed. Semen analysis, determination of serum testosterone level and histopathology of the testes were carried out. Compared to the positive control, the pretreatment by celery oil, vitamin E and their combination for 4 weeks increased the weight of sexual organs, sperm motility, count and viability and serum testosterone level and alleviated histopathological lesions. In conclusion, the pretreatment by celery oil, vitamin E and their combination produced a relative protective effect against reproductive toxicity-induced by SVP in rats. This study recommends that intake of celery oil and vitamin E may be beneficial for patients who suffer from infertility