Effect of some technical processes on deep-freezing, enzyme leakage and fertility of buffalo spermatozoa

ABSTRACT

The present study was performed to clarity the effect of 2 diluents [Tris and Milk], 2 cooling rates [35°C to 5°C for 1 and 2 hr], 2 equilibration periods [1 and 2 hr] and their interactions on the freezability, alkaline phosphatase [AKP], Glutamic oxaloacetic transaminase [GOT] leakages and fertility of frozen-thawed semen in 24 ejaculates from 4 buffalo bulls. The present study revealed that the pre- and post-freezing motility, acrosomal integrity, AKP, GOT leakages and fertility rate were significantly [P<0.01] influenced by different cooling rates and equilibration periods, diluents and their interactions. The mean pre-freeze and post-thawing motility following 2 hr of cooling was significantly higher [P<0.01] than that following 1 hr Whereas, the mean pre-freeze and post-thawing acrosomal defects following 2 hr of cooling was significantly less [P<0.01] than that following 1 hr. The leakage of GOT was significantly lower [P<0.01], leakage of AKP was significantly higher [P<0.01] and the conception rates were higher for semen frozen after 2 hr [64.8%] than from 1 hr [38.4%]