Production and purification of a protease from an alkalophilic Bacillus sp. 2-5 strain isolated from soil
IJB-Iranian Journal of Biotechnology. 2007; 5 (2): 110-113
em Inglês
| IMEMR
| ID: emr-112582
ABSTRACT
This research has focused on isolation and characterization of a strain of Bacillus sp. from alkaline soil, which was able to produce extracellular alkaline protease at pHs ranging from 8 to 11 and temperatures of 20 to 50§C. Also the impact of different carbon and nitrogen sources were investigated. The yield and fold of enzyme purification was 24% and 50 times, respectively. Molecular weight of purified enzyme was measured by SDS-PAGE as 24.7 kDa. The alkaline protease produced by Bacillus sp. 2 - 5 showed the most caseinolytic activity [without any gelatinolytic activity] at pH>10
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Índice:
IMEMR (Mediterrâneo Oriental)
Assunto principal:
Endopeptidases
/
Solo
Idioma:
Inglês
Revista:
Iran. J. Biotechnol.
Ano de publicação:
2007
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