use of hen's egg test -chorioallantoic membrane as a model to evaluate the potential eye irritation of herbicide [Tribenuron-Methyl]

ABSTRACT

The aim of the present study was to evaluate the potential eye irritation of herbicide [tribenuron-methyl] at concentrations [0.01%, 0.02% and 0.03%, w/v] by using the Hen's Egg Test -Chorioallantoic membrane test [HET-CAM] test. According to the our results tribenuron-methyl was classified as non-irritant to eye at concentration [0.01%, w/v] that is recommend by manufacture of herbicide because its doesn't induced any visible changes in the chorioallantoic vessel like [Hyperemia, Hemorrhage, Clotting] thus, the total scores of irritation were [0]. As well as, the increasing concentrations of herbicide at [0.02%, 0.03%, w/v] doesn't induced any vascular injury in [CAM]. In contrast, the N 0.1 NaOH [Positive control] was classified as strongly irritant because its was induced intense hemorrhage with opaque on [CAM] thus, the total scores of irritation were increased to [14]. Physiological saline [0.9% NaCl] [Negative control] doesn't induced any irritant effects in the chorioallantoic vessel thus; the total scores of irritation were [0] and classified as non - irritant for eye. We conclude that, the [HET-CAM] test has been shown to be most suitable for use as a model for evaluating the potential eye irritation of herbicide due to its sensitivity, rapidity and inexpensive as well as reduces the requirement for laboratory animals