Association of C609T and C465T polymorphisms of NQO1 gene with AML

ABSTRACT

Many studies have demonstrated that polymorphisms of NQO1 including C465T and C609T are associated with increased risk of acute myeloid leukemia [AML]. Our aims are to assess incidence of these polymorphisms in Tehran patients and study the influence of low activity of NQOl in AML. In this case-control study, we used PCR and RFLP analyses to study the prevalence of C609T NQO1 in 140 patients, and C465T NQO1 in 124 patients; there was also a control group of 80 being age-sex matched. We calculated odd ratio with SPSS 16 to examine if these polymorphisms are associated with AML. No significant association between the two common polymorphisms of NQO1 and risk of AML was observed. C609T odd ratio for TT genotype versus CC was obtained to be 0.91 [CI 95% = 0.51-1.63] and for CT versus CC it was 1.06 [CI 95% = 0.57-1.95]. C465T odd ratio for TT genotype versus CC was calculated to be 0.22 [CI 95% = 0.009-5.56] and for CT versus CC it came out to be 3.01 [CI 95% = 0.63-14.32]. Our findings suggest that the NQO1 C609T and C465T gene variants do not have a major influence on the susceptibility to adult AML