Studies on the immunizing effect against trichinella spiralis infection in mice using partially purified larval antigen

ABSTRACT

The soluble portion [S3] of large particle fraction of Trichinella spiralis infective larvae was chromatographed on sephacryl S-300 yielding six peaks. Antigens were injected into mice together with Freund's complete adjuvant for three consecutive injections than all mice were challenged with 200L1 larvae orally. Control groups of both mice injected with buffer containing adjuvant and nonimmunized infected mice were used. The influence of immune response on mice was determined by different procedures. Results showed that immunization with S3 and peak four resulted in marked resistance against infection with T. spiralis while the other peaks had no effect