[ determination of bio-kinetic coefficients of crude oil Biodegradation using pseudomonas aeruginosa bacteria]

ABSTRACT

Oil pollution can be generated as a result of spillage, leakage, discharge, exploration, production, refining, transport and storage of crude oil and fuels in the environment. Consequently, many researchers have developed and studied the chemical, physical and biological methods to degrade crude oil. Among them, the biological treatments are the most interesting as they are simple and economical methods. The aim of this study was to determine biokinetic coefficients of crude oil degradation by pseudomonas aerogenusa. This microorganism was isolated in our previous work. In this study the bio-kinetic coefficients of crude oil biodegradation were evaluated. Pseudomonas aerogenusa bacteria which had been isolated from the soil sample taken from a gas station in our previous work were used in this study. This microorganism was cultured in the liquid medium containing crude oil as sole carbon source. Finally with determining the amount of microorganisms and crude oil concentration during biodegradation process, the bio-kinetic coefficients based on modified Monod equation were calculated. Bio-kinetic coefficients obtained from laboratory studies are vital factors in industrial applications. As a result, the bio-kinetic study was performed to find bio-kinetic coefficients for biodegradation of crude oil using the isolated bacteria. The results showed that,Y, k and were equal 0.107, 0.882, 9.39 and 169.3 respectively. Our results showed that Pseudomonas aerogenusa is usable for treatment of oily wastewaters in the full scale facility. Results of this study indicated bio kinetics confections