Frequency of infected bile in patients of uncomplicated gallstone disease

ABSTRACT

To find out the association of bile infection with uncomplicated gallstone disease in our setup. A case control study. This study was carried out at Surgical Department Combined Military Hospital, Multan from 7th March 2007 to 15th September 2007. A total of 100 patients who underwent cholecystectomy for uncomplicated symptomatic cholelithiasis and 40 controls who underwent laparotomy and had no gallbladder stone shown by ultrasound scan, were included. Symptoms, clinical signs, findings of investigations, details of treatment and postoperative care were noted. Main focus of the study was aspiration of bile for culture peroperatively. The bile was sent for culture to assess microflora and their antibiotic susceptibility. The procedures were undertaken under sterile conditions. Out of 100, 30 bile culture positive cases for different micro-organisms were detected. Escherichia coli was found to be the commonest organism in our study [40%]. Next most common organism isolated was Klebsiella pneumoniae [23%]. There were no bacterial isolates in the bile of controls. The study demonstrated that patients with gallstone disease exhibit bactibilia in contrary to patients without gallstones who did not have bacteria in their bile, showing an association between gallstone formation and the presence of bacteria in bile